The MTT assay is a colorimetric assay for assessing cell metabolic activity. [ 1 ] [ 2 ] NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present.

The MTT assay for cellular metabolic activity is almost ubiquitous to studies of cell toxicity; however, it is commonly applied and interpreted erroneously. We investigated the applicability and limitations of the MTT assay in representing treatment toxicity, cell viability, and metabolic activity.

The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity.

2024年6月14日 · The MTT assay, abbreviated “3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide” assay, is a colorimetric assay frequently used to evaluate cell viability and proliferation. It is commonly used to investigate the impact of different substances, medications, or experimental settings on cells health because it offers valuable data ...

2021年7月22日 · Detailed instructions on reagent preparation and assay protocol for an MTT assay to measure cell proliferation or cell cytoxicity.

The MTT assay is defined as a method that measures cellular metabolic activity by assessing the reduction of MTT to purple formazan crystals primarily by mitochondrial dehydrogenases, providing insights into cytotoxicity levels in cells.

2011年1月1日 · The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity.

The MTT assay involves the conversion of the water-soluble yellow dye MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to an insoluble purple formazan by the action of mitochondrial reductase.

Active mitochondria convert the yellow substrate 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to formazan, a purple product. This colorimetric change can be quantified using spectrophotometry and correlated with cell number (Denizot and Lang, 1986).

Metabolic activity: Calorimetric assays that measure metabolic activity are suitable for analyzing cell proliferation, cell viability and cytotoxicity. The reduction of tetrazolium salts such as MTT, XTT and WST-1 to colored formazan compounds occurs only in metabolically active cells. The presence of ATP is also an indicator of metabolic activity.