Single-cell RNA counting at allele and isoform resolution using Smart-seq3
2020年5月4日 · Here we introduce Smart-seq3, which combines full-length transcriptome coverage with a 5′ unique molecular identifier RNA counting strategy that enables in silico …
你要的单细胞全长转录本建库方案：smart-seq3来了 - 简书
时隔7年，2020年5月6日，Rickard团队在smart-seq2的基础上，通过改进开发了smart-seq3技术（Single-cell RNA counting at allele and isoform resolution using Smart …
Smart-seq3 Protocol
2020年2月19日 · After generating fastq files, the zUMIs pipeline should be used to process Smart-seq3 data to ensure correct handling of UMI reads and internal reads. We recommend the …
Smart-seq3xpress：更低成本、更高通量的单细胞测序技术
基于SMART（Switching mechanism at the 5′ end of the RNA template）技术的Smart-seq2是主要的单细胞测序技术之一，具有高灵敏度、全长转录本覆盖度、可检测到稀有转录本等特点，应 …
Single-cell RNA counting at allele and isoform resolution using Smart-seq3
Here we introduce Smart-seq3, which combines full-length transcriptome coverage with a 5' unique molecular identifier RNA counting strategy that enables in silico reconstruction of …
Scalable single-cell RNA sequencing from full transcripts with Smart ...
2022年5月30日 · Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart …
Smart-seq3 - Sandberg Lab
We have integrated Smart-seq3 data processing capabilities into zUMIs (Parekh et al. 2018) to correctly parse molecular and cellular barcodes in an efficient analysis pipeline. The …
Single-cell sequencing of full-length transcripts and T-cell …
2024年11月21日 · We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, …
Single-cell RNA sequencing at isoform resolution - Nature
2020年5月19日 · SMART-seq3 reconstructs the full-length transcripts of single cells with high sensitivity. Technologies to sequence the few hundred attomoles of messenger RNA (mRNA) …
Smart-seq3xpress：更低成本、更高通量的单细胞单细胞全长转录 …
2024年2月7日 · Smart-seq3是最近发展的单细胞转录组测序新方法，引入分子编码对mRNA进行定量统计，使转录本长度增加，灵敏度进一步提高，但仍然无法同时满足高通量、高基因检出能 …