There are an increasing number of recombinant antibodies and proteins in preclinical and clinical development for therapeutic applications. Mammalian expression systems are key to enabling the production of these molecules, and Chinese hamster ovary (CHO) cell …

2021年11月23日 · Mammalian cell-based expression systems are the dominant technology used in the production of biopharmaceuticals. These expression systems excel in terms of protein folding and post-translational modifications, often crucial for the activity and therapeutic effectiveness of recombinant proteins.

Today, there are wide selections of expression systems available for large-scale recombinant protein production. These expression systems include E. coli, baculovirus-mediated insect cell expression, yeast, and several mammalian based systems.

Thermo Fisher Scientific offers the largest collection of mammalian expression vectors, specialized media, delivery reagents, and cells for efficient expression, selection, and analysis of recombinant proteins. There are many options for choosing a mammalian expression system matched to specific needs.

2023年12月15日 · By answering four key questions, readers can determine the most suitable gene expression system following a decision scheme. This guide addresses the most commonly used and accessible systems and provides brief descriptions of the main gene expression systems’ key characteristics to assist decision making.

2023年5月4日 · Mammalian cell-based expression systems are the most common technique used in the production of biopharmaceuticals. These expression systems function remarkably well regarding post-translational modifications and protein folding, which are crucial for recombinant proteins' activity and therapeutic effectiveness.

2017年6月6日 · Mammalian cell culture expression systems rely on the introduction of vector DNA encoding the recombinant protein into a host cell line and harnessing the synthetic capacity of the cell to express and secrete the encoded protein into the cell culture medium.

Translation or protein synthesis is a multi-step process that requires macromolecules like ribosomes, transfer RNAs (tRNA), mRNA and protein factors as well as small molecules like amino acids, ATP, GTP and other cofactors. There are specific protein factors for each step of translation (see table below).

2016年5月11日 · Expression of proteins in mammalian cells is a key technology important for many functional studies on human and higher eukaryotic genes. Studies include the mapping of protein interactions, solving protein structure by crystallization and X-ray diffraction or...

Learn how to choose the best mammalian expression system for your specific application from our selection of pCMV-based vector systems, which incorporate HA-, or Myc-fusion tags. All of these systems provide the following advantages: