Lactobacillus crispatus DSM 20584 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from eye. Gram-positive; rod-shaped; anaerobe; mesophilic; 16S sequence; Bacteria; genome sequence

2015年4月28日 · Lactobacillus spp. are facultatively anaerobic, catalase-negative, Gram-positive, non-spore-forming rods that often grow better under microaerophilic conditions. Their Gram stain morphology can vary, including as short, plump rods, long, slender rods, in chains or palisades.

In line with previous findings, the relative and absolute proportion of L. crispatus, a hallmark of vaginal eubiosis, inclined to decrease in the transition from H to BV conditions (Ceccarani et...

2019年5月7日 · In this study, we investigated the therapeutic effects and mechanism of Lactobacillus crispatus or delbrueckii on vaginitis caused by Candida albicans in a Sprague–Dawley rat model. A microbiological evaluation was performed by Gram staining and fungal colonies were enumerated.

Morphological characterization was studied through the gram staining technique by Hans Christian Gram . The catalase test [ 38 ], Simmons citrate test [ 39 , 40 ], methyl red and Voges–Proskauer's test [ 41 ], indole production test [ 40 , 42 ], and oxidation fermentation test [ 43 , 44 ] were performed for biochemical characterization of the ...

Burrillo et al. have recently devised an adaptation to the Gram staining method used to identify bacteria, by coupling this with a matrix-assisted laser desorption ionization–time of flight mass (MALDI-TOF) spectrometric method, which is able to detect the presence and type of bacteria present in urine [91].

Live L. crispatus L1 cells strongly reduced adhesion of a yeast pathogenic strain, Candida albicans in particular, in adherence assays. Interestingly a higher expression of the human defensin HBD-2 was also observed in vaginal cells treated with the purified exopolysaccharide, indicating a possible correlation with C. albicans growth inhibition.

2012年9月21日 · For each woman, Gram stains were scored, the quantitative changes of 5 key vaginal species, i.e. Atopobium vaginae, Lactobacillus crispatus, L. iners, (sialidase positive) Gardnerella vaginalis and Prevotella bivia were quantified with qPCR and hydrogen-peroxide production was assessed on TMB+ agar.

In this study, we aim to identify factors confirming the Gram-negative stain appearance of L. iners by uncovering the ultrastructure of its cell envelope using transmission electron microscopy...

Exposing infected human BECs to L. crispatus in vitro markedly reduced the intracellular uropathogenic Escherichia coli (UPEC) load. The adherence of Lactobacilli to BECs was found to result in increased type I interferon (IFN) production, which in turn enhanced the expression of cathepsin D within lysosomes harboring UPECs.