2023年12月15日 · To generate NEUROG1/2 induced neurons (iNs), on day 0, NYGCe001-A cells were plated in the presence of 1 μg/ml doxycycline (Sigma) in Essential 8 medium. The cells …

2021年5月25日 · Results showed that doxycycline induced neurite outgrowth via activation of the trkA receptor and the downstream signaling pathways, PI3K/Akt and MAPK/ERK, without …

To generate NEUROG1/2 induced neurons (iNs), on day 0, NYGCe001-A cells were plated in the presence of 1 μg/ml doxycycline (Sigma) in Essential 8 medium. The cells were plated in …

Here, using inducible expression of NEUROG1 and NEUROG2, we identify transcription factors (TFs) required for directed neuronal differentiation by combining expression and chromatin …

2023年2月27日 · Doxycycline reduced the inhibition of neuritogenesis induced by MPP +, even in cells deprived of NGF. The mechanism involved the upregulation of GAP-43, synapsin I, β-III …

2008年1月1日 · In the Tet-Off system, the tTA proteins in the absence of doxycycline (Dox), the analog of Tet, induce target gene expression through binding of tTA homodimers to tetO …

1998年8月1日 · Using calcineurin as a test transgene, we show that doxycycline-induced expression impairs both an intermediate form of LTP (I-LTP) in the hippocampus and the …

2019年7月1日 · By using a DOX-inducible Tet-ON system, we identified that DOX alone dramatically promoted dopaminergic (DA) neuron differentiation from human pluripotent stem …

Here I demonstrate the generation of hiPSCs of BD patient and control genetic backgrounds, that are capable of doxycycline-inducible differentiation to both excitatory and inhibitory neuron …

2023年9月2日 · After doxycycline (Dox) addition, ESCs are rapidly converted to induced neurons: Ascl1 and Ngn2 produce cells expressing the neuronal marker TUBB3 and displaying …