Collagen I存在翻译后修饰，一部分修饰参与胶原纤维合成，另一部分如异构化、外消旋化、糖基化等修饰，会改变Collagen I的生物学功能，其终产物已被证明为一些疾病的生物标志物。

Collagen靶点介绍 蛋白功能. 胶原蛋白（Collagens）是人体最富集且非常重要的蛋白，其最常见形式为I型、II型、III型、IV型和V型。 胶原蛋白是细胞外基质的主要组成成分，可以促进细胞粘附和迁移。

Collagen I 是骨骼、皮肤及肌腱中胞外基质的主要蛋白组分，主要由成骨细胞、真皮层纤维状细胞 及肌腱细胞分泌而来。 合适的胶原分离提取方法对于Collagen I 的WB 实验成功至关重要。

Human Collagen Type I ELISA kit (ab285250, E4617) is a sandwich ELISA assay for the quantitative measurement of COL1 in human serum, plasma, cell culture supernatants, cell lysates, and other biological fluids. The density of color is proportional to the amount of COL1 captured from the samples.

Collagen I antibody (Collagen 1 antibody) detects type I collagen, a fibrillar type collagen found in almost all connective tissues. The main isoform of type I collagen is a heterotrimer of two α1 and one α2 chains subject to significant posttranslational modification and crosslinking.

COL1A1/Collagen I Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。 Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs.

Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex composed of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen) (1-3).

No significant cross-reactivity or interference between Mouse COL1(Collagen Type Ⅰ) and analogues was observed. 重复性 三种不同浓度的样本在同一块板子上分别检测20次，以及在不同的板子上分别检测20次，板内和板间变异系数均小于10%。