What was needed was a simple quantitative assay for this enzyme. With this in mind, I developed a spectrophotometric assay to purify rabbit lung ACE (ref. 5) and to study its enzymatic properties ...
As p-nitrophenol is a skin irritant, you should wear gloves throughout these assays. 2. Prepare a sub-stock by diluting 100 µl of original ... 5.5 mM). 5. Now you have to determine enzyme activities ...
When this region was mutated, the enzyme lost its ability to form this dimeric structure. The findings were published in the journal Nature Communications. Functional assays showed that the enzyme ...