We contend that microfluidic droplets offer a tractable platform for developing and standardizing a single-cell enzyme activity assay specifically adapted to handle the high diversity and ...

and functional assays. Applied to amide synthetases, this approach evaluated 1217 variants across 10,953 reactions, successfully predicting optimized enzymes for synthesizing nine pharmaceutical ...

In a study published in Nature, researchers report the successful in vitro reconstitution of meiotic DNA double-strand breaks ...

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.langmuir.4c05114.

Absorbance was measured at 290 nm. CHS, CHI, F3’H, and other enzyme activity assays were performed according to the kit, and enzyme levels were determined by ELISA. 2.4 RNA extraction and ...

SignalChem Biotech, part of Sino Biological, offers one of the largest active enzyme collections in the world and specializes ...

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia ...

“To address this challenge, we develop a machine learning (ML)-guided platform that integrates cell-free DNA assembly, cell-free gene expression, and functional assays to rapidly map fitness ...

Oral contraceptive pills (OCPs) are commonly used for contraception, but their long-term effects on oxidative stress, lipid profiles, and liver function remain unclear. This study aimed to evaluate ...