CRISPR-Cas系统是源自细菌和古细菌的适应性免疫系统，用以抵御病毒和其他外来遗传物质的入侵。该系统的运作分为三个主要阶段：间隔区序列的获取 ...

CRISPR/Cas systems are widespread RNA-mediated prokaryotic adaptive immune systems providing protection against invading nucleic acids 1. However, throughout evolution, this host defense system ...

2020年，詹妮弗·杜德纳（Jennifer A. Doudna）教授与埃马纽埃尔·夏彭蒂耶（Emmanuelle Charpentier）共同荣获诺贝尔化学奖，以表彰她们共同开发了基因组编辑方法CRISPR的卓越贡献。 受Frontiers for Young Minds邀请，杜德纳撰文解释了CRISPR的工作原理以及她与合作者是如何发现这一机制的。杜德纳说， ...

Biomedical engineers at Duke University have demonstrated a promising new approach that could be used to treat a rare and ...

It happened probably in 2007 when a paper appeared in Science, describing for the first time the possible function of the CRISPR-Cas system as an antiviral defense system in bacteria. And we ...

researchers soon realized that they could harness this system for gene manipulation in any cell. All they needed to do was design a CRISPR gRNA sequence that bound to a specific DNA sequence and ...

5,15 Both technologies are based on the CRISPR-Cas system but utilize different CRISPR-associated proteins, Cas13 for SHERLOCK and Cas12a for DETECTR, to achieve their highly specific diagnostic ...

A UCO team has applied the genome editing technique, which garnered a Nobel Prize in Chemistry in 2020, to unravel the ...

为了解决CRISPR-Cas基因组编辑中的一个根本性局限，研究人员研发了新型的经设计的Cas9变体，后者几乎消除了对一个被称为PAM的原间隔序列相邻基序 ...

This study presents an optimized CRISPR-Cas12a system for large-scale genetic screening, revealing critical genes involved in ...

Potent in vivo gene editing in skeletal muscle of non-human primates by a novel, ultracompact CRISPR system delivered via a single AAV vector.