Although this step is what gives the technique the name "western blotting," the term is typically used to describe the entire procedure. Once the transfer is complete, the membrane carries all of ...

Use alkaline transfer buffer (0.4 M NaOH with 1 M NaCl) for charged nylon membranes. 9| Place a piece of thick blotting paper on a sheet of Plexiglas or a glass plate to form a support that is ...

The main steps in a western blot protocol are as follows. Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins ...

It is important to understand the advantages and disadvantages of different ways of detecting a protein of interest on a western blot membrane. Here, we consider the three main types ... antibody ...

The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

Are you looking for an all-in-one solution for your lab's imaging needs? ProteinSimple's FluorChem imagers enable high-quality multimodal imaging of your Western blot membranes and electrophoresis ...