The use of nickel in immobilized metal affinity chromatography, as during isolation of His-tagged proteins, can hinder the purification of recombinant proteins. To circumnavigate this limitation ...

Researchers have produced a review of chromatography media and purification processes for handling complex and super-large ...

They are very simple to use and are ideal for inexperienced chromatography researchers. The spin columns allow highly specific binding and rapid protein purification with often >90–95% purity.

The use of electrical impulses also speeds up the purification process, Ferrand-Drake del Castillo says, explaining that “instant millisecond switches reduce the cycle time and use of raw materials by ...

With a deep dive into the implementation of high-capacity resins and continuous chromatography, discover how to enhance purification ... of next generation Protein A resins in large-scale ...

Increase in R&D expenditure in biopharmaceuticals, rise in demand for biologics and monoclonal antibodies (mAbs), and rise in adoption of biosimilars are the major factors that drive the growth of the ...

for purification. The DNA library was generated using PCR, Gibson assembly, and iterative site saturation mutagenesis, with ML predictions refining McbA variants. Expression was performed in E. coli ...