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生物通
1 年
普洛麦格中文通讯 第八期 2003
表1显示了用不同的退火DNA寡聚核苷酸为模板合成siRNA或发夹siRNA的产率,一般而言,每毫升反应体系中,siRNA产量>500μg,发夹siRNA的产量为1mg。对任何一种特异的模板,使用者可通过增加孵育时间(从30分钟到2小时)或提高转录反应温度(用42℃替代37℃) 来提高 ...
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