CRISPR/Cas systems are widespread RNA-mediated prokaryotic adaptive immune systems providing protection against invading nucleic acids 1. However, throughout evolution, this host defense system ...

Beans are a key food at the dietary level, boasting high nutritional value and constituting the most directly consumed legume ...

该研究对用于 CRISPR 基因编辑治疗的 Cas 核酸酶 SaCas9 和 AsCas12a 进行了理性工程化改造，设计出了具有最小化免疫原性的 SaCas9 和 AsCas12a 突变体。

A UCO team has applied the genome editing technique, which garnered a Nobel Prize in Chemistry in 2020, to unravel the ...

researchers soon realized that they could harness this system for gene manipulation in any cell. All they needed to do was design a CRISPR gRNA sequence that bound to a specific DNA sequence and ...

为了解决CRISPR-Cas基因组编辑中的一个根本性局限，研究人员研发了新型的经设计的Cas9变体，后者几乎消除了对一个被称为PAM的原间隔序列相邻基序 ...

This study presents an optimized CRISPR-Cas12a system for large-scale genetic screening, revealing critical genes involved in lymphoma development and therapy.

Australian researchers have successfully introduced an improved version of Cas12a gene-editing enzyme in mice.

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...

Potent in vivo gene editing in skeletal muscle of non-human primates by a novel, ultracompact CRISPR system delivered via a single AAV vector.

Complementary discoveries have the potential to enhance treatment options for antibiotic-resistant infections. Researchers at ...